Selective staining of immature hemopoietic cells with merocyanine 540 in flow cytometry
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چکیده
منابع مشابه
Photodynamic action of merocyanine 540 on carcinoma of cervix cells.
Results of the studies carried out on localization and photodynamic action of merocyanine 540 (MC540) on carcinoma of cervix (HeLa) cells are presented. Fluorescence microscopic study showed that when HeLa cells were incubated with MC540 in dark, the dye localized in plasma membrane of cells. Photoirradiation of cells in presence of MC540 led to enhancement of dye uptake, intracellular localiza...
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Merocyanine 540 (MC540) is a fluorescent probe that binds preferentially to membranes with loosely packed lipids. When combined with flow cytometry, it provides a novel methodology for rapidly and quantitatively assessing lipid organization in individual leukocytes. Analysis of cells stained simultaneously with MC540 and 1-[4-trimethylammoniumphenyl]-6-phenyl-1,3,5-hexatriene, to normalize for ...
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The aim of this study was to determine whether flow cytometric evaluation of combined merocyanine 540 and Yo-Pro 1 (M540-YP) staining would identify viable dog sperm that had undergone membrane stabilization known to be associated with capacitation in other species, and whether such destabilization is detected earlier than when using the tyrosine phosphorylation and ethidium homodimer (TP-EH) s...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1988
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990090104